RRML - Expression profiles of immunomodulatory genes in human solid tumors
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English

Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12

Advanced search

Top 10 downloaded articles
- December 2024 -
 
Biomarkers of acute kidney inj... 9
Investigation of cytokine chan... 9
A comprehensive review of glyc... 8
Recomandările naționale ale ... 8
Towards appropriate training f... 8
Romanian Review of Laboratory ... 6
Validation of GOD / PAP method... 5
Expressions of vascular endoth... 5
Setting up an own laboratory p... 3
The frequency of EGFR gene mut... 3

Log in

Concept, Design & Programming
Dr. Adrian Man
   
 
Nr. 19(1)/2011
XML
TXT

Expression profiles of immunomodulatory genes in human solid tumors

Ildikó Mészáros, Tibor L. Krausz, Eva Fischer-Fodor, Szabolcs Lányi


Abstract:

Due to antigen recognition, effector and regulatory function, lymphocytes have pivotal function in many disease pathways. Immune response towards foreign or altered self antigens has a considerable complexity, the fine modulation of the immune response being delivered by the interaction between costimulatory molecules, on which the final outcome of the immune response depends. Evaluation of the expression profile of immunomodulatory genes and cytokines in cancer may clear some of this complexity. Hence we used a PCR screening method in order to assess mRNA presence and the quantity of relative genes in neoplastic pathologies. We proposed to establish standard “yes or no” condition to the PCR method in order to confer a semi-quantitative attribute to our PCR. We used primers for the following genes: GITR, GITRL, IL-2, CD25, CD28, CTLA-4, TGF β1, IL-18 and GAPDH, the latter one as internal control. We collected samples from patients with mammary, kidney, rectal, ovary and colonic solid tumors, from voluntary subjects and from patients with inflammatory pathology used as controls, isolated lymphocytes from blood and tissue, and extracted the total mRNA, followed by reverse transcription to cDNA. We confirmed quality and quantity of the total mRNA using GAPDH. We obtained results demonstrating that there are significant differences in the expression of these genes in the above mentioned patients compared to healthy subjects and inflammatory controls.

Keywords: gene expression,cancer,TGF β1,GITR,GITRL,CD28,CTLA-4,IL-2,IL-2Rα (CD25),IL-18

 
  PDF Download full text PDF
(393 KB)
     
 
How to cite
Mészáros I, Krausz TL, Fischer-Fodor E, Lányi S. Expression profiles of immunomodulatory genes in human solid tumors. Rev Romana Med Lab. 2011;19(1):65-74