RRML - Vascular Endothelial Growth Factor Insertion/Deletion gene polymorphism in patients with type 2 diabetes and diabetic peripheral polyneuropathy

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Nr. 22(2)/2014 DOI:10.2478/rrlm-2014-0023

Vascular Endothelial Growth Factor Insertion/Deletion gene polymorphism in patients with type 2 diabetes and diabetic peripheral polyneuropathy

Adina Stoian, Anca Bacârea, Anca Moţăţăianu, Mircea Stoian, Florina Gliga, Vladimir Bacârea, Duicu Carmen, Claudia Bănescu

Abstract:

The aim of this work was to study for the first time in Romania Insertion/Deletion (I/D) polymorphism of the Vascular Endothelial Growth Factor (VEGF) gene in a group of patients with established type 2 diabetes mellitus (DM) and diabetic peripheral neuropathy (DPN) compared with a control group. This was a case-control study consisting of a group of 84 patients with type 2 DM and DPN, diagnosed by clinical neurological examination and electrophysiological nerve conduction studies and a control group of 90 healthy volunteers. For deoxyribonucleic acid (DNA) isolation, a DNA purification kit from Zymo Research was used. In vitro amplification of DNA sequences was achieved by polymerase chain reaction (PCR). Selective in vitro amplification of a DNA fragment of known sequence is based on the principle of extension of a primer (“primer and PCR amplicon”). DNA fragments were separated by gel electrophoresis. For proper viewing and interpreting of agarose gels Vilbert Lourmat system was used. D allele frequency of VEGF was significantly higher in patients with diabetic peripheral neuropathy (53.57%) compared with controls (25%), p=0.0001. There is a positive association between I/D polymorphism of VEGF gene and the presence of diabetic peripheral polyneuropathy. Our study suggests that D allele of VEGF gene is a risk factor for the occurrence of DPN.

Keywords: polyneuropathy, I/D polymorphism, VEGF gene, diabetes mellitus

Received: 17.6.2013
Accepted: 17.3.2014
Published: 25.5.2014

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Stoian A, Bacârea A, Moţăţăianu A, Stoian M, Gliga F, Bacârea V, et al. Vascular Endothelial Growth Factor Insertion/Deletion gene polymorphism in patients with type 2 diabetes and diabetic peripheral polyneuropathy. Rev Romana Med Lab. 2014;22(2):165-72. DOI:10.2478/rrlm-2014-0023