RRML - Serum cytokine and chemokine profiles of patients with confirmed bacterial and viral meningitis
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English

Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12

Advanced search

Top 10 downloaded articles
- May 2024 -
 
Diagnostic value of stress myo... 4
Effect of Systemic Inflammator... 4
Monitoring the sensitivity of ... 3
A comprehensive review of Prof... 3
Evaluation of routine laborato... 3
The role of nitro oxidative fa... 3
Correlations of expressions of... 3
Evaluation of cytotoxicity and... 3
Clinical, laboratory and molec... 1
Prader–Willi Syndrome and di... 1

Log in

Concept, Design & Programming
Dr. Adrian Man
   
 
Nr. 31(4)/2023 DOI:10.2478/rrlm-2023-0023
XML
TXT

Research article

Serum cytokine and chemokine profiles of patients with confirmed bacterial and viral meningitis

Ramona Caragheorgheopol, Cătălin Țucureanu, Veronica Lazăr, Iuliana Caraș

Correspondence should be addressed to: Ramona Caragheorgheopol

Abstract:

Introduction: Cerebrospinal fluid (CSF) cytokines and chemokines have been reported by several studies as useful markers to discriminate bacterial and viral meningitis (BM and VM). This study aimed to investigate if serum cytokine and chemokine profiles could also differentiate BM from VM, thus circumventing the need for an invasive lumbar puncture. Methods: Serum cytokines and chemokines were measured in 153 samples from patients with BM (n=58), VM (n=69), and controls (C, n=26) using multiplex assays. Cytokine and chemokine concentrations were compared among groups, correlation analyses were performed, and BM and VM cases classification based on cytokine and chemokine patterns was tested using a Machine Learning algorithm. Results: IL-8, IL-1β, IL-6, IL-10, TNF-α, MCP-1, and ENA-78 showed a pronounced increase in the BM group compared to C (P<0.01). Comparison of cytokines and chemokines in BM vs. VM showed significantly higher levels of MCP-1, IL-8, IL-1β, IL-6 and IL-10 (P<0.01). Serum cytokine and chemokine concentrations were highly correlated in BM, being strongest for: MCP-1/IL-8, MCP-1/IL-1β, and IL-8/IL-1β (r=0.83; r=0.72; r=0.78, respectively). In VM, cytokine and chemokine correlations were weaker. The best predictors in the cytokine and chemokine pattern identified with a Random Forest algorithm for classifying BM vs VM were IL-8 and IL-10, and IL-6, but the specificity and sensitivity were low (85% and 69%, respectively). Conclusion: Our results suggest significant changes in serum IL-6, IL-8, IL-10, and IL-1β in BM, but these mediators may have limited value in differentiating BM from VM.

Keywords: machine learning, random forest, meningitis diagnosis, differential cytokine pattern, serum cytokines chemokines

Received: 20.4.2023
Accepted: 27.5.2023
Published: 24.8.2023

 
  PDF Download full text PDF
(951 KB)
  PDF Supplementary material
     
 
How to cite
Caragheorgheopol R, Țucureanu C, Lazăr V, Caraș I. Serum cytokine and chemokine profiles of patients with confirmed bacterial and viral meningitis. Rev Romana Med Lab. 2023;31(4):315-24. DOI:10.2478/rrlm-2023-0023