RRML - Detection of mecA gene in clinical Staphylococcus aureus isolates from Infectious Diseases Hospital, Iasi, Romania
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English


Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12


Advanced search


Top 10 downloaded articles
- June 2024 -
 
The evaluation of a diagnostic... 10
A comprehensive review of Prof... 9
Assessment of ocrelizumab impa... 7
Romanian Review of Laboratory ... 6
Monitoring the sensitivity of ... 6
Challenges in prophylactic the... 6
Diagnostic value of stress myo... 6
Effect of Systemic Inflammator... 5
Evaluation of routine laborato... 4
The role of nitro oxidative fa... 4

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 19(3)/2011
XML
TXT

Detection of mecA gene in clinical Staphylococcus aureus isolates from Infectious Diseases Hospital, Iasi, Romania

Olivia S. Dorneanu, Teodora Vremeră, Eduard V. Năstase, Cătălina Logigan, Aida C. Bădescu, Egidia G. Miftode


Abstract:

Introduction. Since it was first described in 1961, methicillin-resistant Staphylococcus aureus (MRSA) has been an increasingly important cause of health care-associated infections worldwide. During the 1990s, new strains of MRSA emerged and caused community-associated infections on multiple continents. Methods. We have investigated 151 clinically significant S. aureus strains isolated from blood cultures, cerebro-spinal fluid, pus, sputum samples collected from patients with infections admitted to the Infectious Diseases Hospital “Sf. Parascheva” Iaşi, Romania, between January 2008 and December 2009. We have performed real-time PCR for mecA gene detection, disk diffusion susceptibility testing and minimum inhibitory concentration determination to anti-staphylococcal antibiotics for all isolates. Results. The oxacillin resistance rate was 45.7%. All MRSA strains were susceptible to vancomycin, linezolid and teicoplanin; however, they exhibited high rates of resistance to erythromycin (70.5%), tetracycline (67.6%), gentamicin (48.5%) and ciprofloxacin (44.1%). Susceptibility rate to trimethoprim/sulfamethoxazole in MRSA strains was 89.7%. Almost a third of the tested strains had oxacillin MICs 256 µg/ml. For 90% of the strains vancomycin MICs was 2 µg/ml. Conclusions. Glycopeptides remain the first choice therapy for MRSA infections. To preserve their value, their use should be limited to those cases where they are clearly needed.

Keywords: Staphylococcus aureus,mecA,resistance

 
  PDF Download full text PDF
(155 KB)
     
 
How to cite
Dorneanu OS, Vremeră T, Năstase EV, Logigan C, Bădescu AC, Miftode EG. Detection of mecA gene in clinical Staphylococcus aureus isolates from Infectious Diseases Hospital, Iasi, Romania. Rev Romana Med Lab. 2011;19(3):259-65