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Research article Correlations of expressions of Toll-like receptor 4 and Janus kinase 3 in peripheral blood mononuclear cells with T helper 17 cell/ regulatory T cell imbalance in patients with ankylosing spondylitis Ruyan He, Guangpu Liu, Fang Gu, Junxia Yang, Meirong Li
Correspondence should be addressed to: Fang Gu
Abstract: Background: We aimed to analyze the correlations of the expressions of Toll-like receptor 4 (TLR4) and Janus kinase 3 (JAK3) in peripheral blood mononuclear cells (PBMCs) with T helper 17 cell (Th17)/ regulatory T cell (Treg) imbalance in patients with ankylosing spondylitis (AS). Methods: A total of 101 AS patients were enrolled into an AS group. Based on the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) score, they were subdivided into a stable AS (ASS) group (BASDAI score <4 points, n=42) and an active AS (ASA) group (BASDAI score ≥4 points, n=59). Meanwhile, 50 healthy volunteers who received health examination in the same time period were assigned into a healthy control (HC) group. RT-qPCR was performed to determine the mRNA expressions of TLR4 and JAK3 in PBMCs. Enzyme-linked immunosorbent assay was conducted to measure the levels of Th17/ Treg-related cytokines [interleukin (IL)-10, IL-17, IL-23, and transforming growth factor-β (TGF-β)] in the serum. Results: The mRNA expressions of TLR4 and JAK3 had positive correlations with IL-17 (r=0.592, 0.542) and IL-23 levels (r=0.529, 0.590) (P 0.05). The levels of TLR4 mRNA, JAK3 mRNA, IL-17, and IL-23 were all correlated with the occurrence of AS (P<0.05). The areas under the receiver operating characteristic curves of TLR4, JAK3, and their combination in PBMCs for predicting the occurrence of AS were 0.912, 0.835, and 0.919, respectively. Conclusions: TLR4 combined with JAK3 can effectively predict the occurrence of AS. Keywords: ankylosing spondylitis, correlation, expression, mononuclear cell, peripheral blood
Received: 13.10.2023 |
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He R, Liu G, Gu F, Yang J, Li M. Correlations of expressions of Toll-like receptor 4 and Janus kinase 3 in peripheral blood mononuclear cells with T helper 17 cell/ regulatory T cell imbalance in patients with ankylosing spondylitis. Rev Romana Med Lab. 2024;32(2):171-6. DOI:10.2478/rrlm-2024-0016
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