RRML - Fluorescence In Situ Hybridization on peripheral blood is a sensitive and reliable method for evaluation of minimal residual disease in CML

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Dr. Adrian Man

Nr. 20(2)/2012

Fluorescence In Situ Hybridization on peripheral blood is a sensitive and reliable method for evaluation of minimal residual disease in CML

Cerasela Jardan, Dumitru Jardan, Daniel Coriu, Emilia Severin

Abstract:

This study was aimed to investigate the value of detecting BCR/ABL fusion gene by fluorescence in situ hybridization (FISH) on peripheral blood specimens from patients with chronic myeloid leukemia (CML). Mate-rials and Methods: We analyzed 126 samples (83 bone marrow and 43 peripheral blood samples) from patients with CML referred to Fundeni Clinical Institute between 2010 and 2011. Samples were analyzed using interphase FISH on peripheral-blood specimens and the results were compared with those of conventional cytogenetics and interphase FISH on bone marrow. Results: in comparison to conventional cytogenetics and bone marrow FISH, interphasic FISH on peripheral blood did not generated any discrepancies regarding the level of minimal residual disease. Conclusion: In this study we evaluated FISH technique as an alternative and rapid method for monitoring minimal residual disease in CML patients treated with tyrosine kinase inhibitors. FISH on peripheral blood is a molecular cytogenetic technique that allows minimal residual disease monitoring on interphase nuclei. This technique has the advantage of generating results in a short time (even in 24h) and is less invasive than bone marrow biopsy.

Keywords: peripheral blood-FISH technique,BCR/ABL gene fusion,minimal residual disease

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Jardan C, Jardan D, Coriu D, Severin E. Fluorescence In Situ Hybridization on peripheral blood is a sensitive and reliable method for evaluation of minimal residual disease in CML. Rev Romana Med Lab. 2012;20(2):151-6