RRML - Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages

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Nr. 30(2)/2022 DOI:10.2478/rrlm-2022-0015

Research article

Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages

Sibel Köktürk, Yeliz Cetinkol, Selma Cirrik

Correspondence should be addressed to: Sibel Köktürk

Abstract:

Introduction: Crinum latifolium is a natural plant showing the anti-inflammatory effects. We aimed to evaluate the effects of Crinum latifolium extract on proinflammatory cytokine production and maturation of early and late endosomes in the lipopolysaccharide (LPS)-induced human macrophages at light microscopic and biochemical levels. Material and Methods: The effects of Crinum latifolium extract in human macrophages stimulated with LPS was evaluated to optic densities of the lysosome-associated membrane protein-1 (LAMP-1) and early endosome antigen 1 (EEA1) by immunohistochemistry staining and to level of the proinflammatory cytokine interleukin (IL)-6 by enzyme-linked immunosorbent assay (ELISA). Results: Crinum latifolium group exhibited a significant decreased in the levels of IL-6 in the supernatant as compared to the LPS group for 2 and 72 hours (p<0.0001). Crinum latifolium group exhibited a significant increased in the optic density of the EEA1 and LAMP-1 in coverslips as compared to the control and lipopolysaccharide groups after 2 and 72 hours (p<0.0001). Conclusions: Crinum latifolium may a therapeutic plant in the inﬂammatory diseases, such as sepsis, through anti-inflammatory effects, such as decrease in production of the proinflammatory cytokine and increase in maturations of the early and late endosomes in macrophages.

Keywords: inflammation, macrophage, interleukin-6, Crinum latifolium extract, endosomes

Received: 20.11.2021
Accepted: 21.3.2022
Published: 6.4.2022

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Köktürk S, Cetinkol Y, Cirrik S. Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages. Rev Romana Med Lab. 2022;30(2):183-90. DOI:10.2478/rrlm-2022-0015