RRML - Malondialdehyde levels can be measured in biological samples by using a fast HPLC method with visible detection

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Concept, Design & Programming
Dr. Adrian Man

Nr. 24(3)/2016 DOI:10.1515/rrlm-2016-0029

Research article

Malondialdehyde levels can be measured in biological samples by using a fast HPLC method with visible detection

Erzsébet Fogarasi, Mircea Dumitru Croitoru, Ibolya Fülöp, Enikő Nemes-Nagy, Robert Gabriel Tripon, Zsuzsanna Simon-Szabo, Daniela-Lucia Muntean

Correspondence should be addressed to: Mircea Dumitru Croitoru

Abstract:

Oxidative stress appears when the amount of free radicals that are formed in a living organism exceed its spin-trapping ability. One of the most dangerous free radicals that are formed in the human body is the hydroxyl radical. It can alter several biomolecules, including the unsaturated fatty acids; this process is known as lipid peroxidation and can lead to cell necrosis and generation of several harmful byproducts including malondialdehyde, which serves also as a biomarker of oxidative stress. A new HPLC method with visible detection was developed for the detection of malondialdehyde in human serum and saliva samples. The method was verified in terms of specificity, linearity, limits of detection (0.35 ng/ml), limit of quantification (1.19 ng/ml), recovery (90.13±10.25 - 107.29±14.33) and precision (3.84±1.49% - 6.66±1.76%). An analysis time of only 1 minute was obtained and no interferences from the matrices were observed. Statistical analysis (Pearson correlation test) showed a moderate correlation (R = 0.5061, p = 0.0099) between serum and saliva concentrations (N = 25). The possibility of measuring salivary concentrations of malondialdehyde extents the applications of oxidative stress/lipid peroxidation estimations to categories of population unreachable before (pregnant women, small children, etc); repeated sample studies are also easier to make.

Keywords: malondialdehyde, serum, saliva, HPLC

Received: 19.5.2016
Accepted: 17.8.2016
Published: 22.8.2016

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Fogarasi E, Croitoru MD, Fülöp I, Nemes-Nagy E, Tripon RG, Simon-Szabo Z, et al. Malondialdehyde levels can be measured in biological samples by using a fast HPLC method with visible detection. Rev Romana Med Lab. 2016;24(3):319-26. DOI:10.1515/rrlm-2016-0029