RRML - The role of BMP-2 in mouse embryonic stem cells differentiation

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Nr. 20(1)/2012

The role of BMP-2 in mouse embryonic stem cells differentiation

Emoke Pall, Mihai Cenariu, Ioan S. Groza

Abstract:

Embryonic stem cells have the ability to remain undifferentiated and proliferate in vitro while maintaining the potential to differentiate into derivatives of all three embryonic germ layers. Embryonic stem cells (ESCs), isolated from embryos are established as permanent lines with self-renewal and differentiation capacity in various specialized cell lines. We used at our experiment the mouse ES cell line KA1/11/C3/C8 (mouse embryonic stem cells line, gift from, Gocza Elen, Genetic Modification Group, Agricultural Biotechnology Center, Godollo, Hungary) with a normal karyotype, at 16th passages. Because BMP-2 (Bone Morphogenetic Protein - 2) plays a crucial role in the induction of heart formation of vertebrate embryos our study was designed to evaluate the effect of BMP-2 on mouse embryonic stem cells differentiation.

Keywords: embryonic stem cells,in vitro differentiation,cardiac,growth factors

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Pall E, Cenariu M, Groza IS. The role of BMP-2 in mouse embryonic stem cells differentiation. Rev Romana Med Lab. 2012;20(1):39-44