RRML - General Principles of Haematopoetic Stem Cell Transplantation. The Role of the Clinical Laboratory in High Quality Grafts Harvesting

Main page
News
RJLabM Credits
Archive
- Current issue
- Ahead of print
- List of published issues
- 2024
  - Nr.4/2024
  - Nr.3/2024
  - Nr.2/2024
  - Nr.1/2024
  - Supplement 1/2024
- 2023
  - Nr.4/2023
  - Nr.3/2023
  - Nr.2/2023
  - Nr.1/2023
  - Supplement 1/2023
- 2022
  - Nr.4/2022
  - Nr.3/2022
  - Nr.2/2022
  - Nr.1/2022
  - Supplement 1/2022
- 2021
  - Nr.4/2021
  - Nr.3/2021
  - Nr.2/2021
  - Nr.1/2021
- 2020
  - Nr.4/2020
  - Nr.3/2020
  - Nr.2/2020
  - Nr.1/2020
  - Supplement 1/2020
- 2019
  - Nr.4/2019
  - Nr.3/2019
  - Nr.2/2019
  - Nr.1/2019
  - Supplement 1/2019
- 2018
  - Nr.4/2018
  - Nr.3/2018
  - Nr.2/2018
  - Nr.1/2018
  - Supplement 1/2018
  - Supplement 2/2018
  - Supplement 3/2018
- 2017
  - Nr.4/2017
  - Nr.3/2017
  - Nr.2/2017
  - Nr.1/2017
  - Supplement 1/2017
- 2016
  - Nr.4/2016
  - Nr.3/2016
  - Nr.2/2016
  - Nr.1/2016
  - Supplement 1/2016
- 2015
  - Nr.4/2015
  - Nr.3/2015
  - Nr.2/2015
  - Nr.1/2015
  - Supplement 1/2015
- 2014
  - Nr.4/2014
  - Nr.3/2014
  - Nr.2/2014
  - Nr.1/2014
  - Supplement 1/2014
- 2013
  - Nr.4/2013
  - Nr.3/2013
  - Nr.2/2013
  - Nr.1/2013
  - Supplement 1/2013
- 2012
  - Nr.4/2012
  - Nr.3/2012
  - Nr.2/2012
  - Nr.1/2012
  - Supplement 1/2012
- 2011
  - Nr.4/2011
  - Nr.3/2011
  - Nr.2/2011
  - Nr.1/2011
  - Supplement 1/2011
- 2010
  - Nr.4/2010
  - Nr.3/2010
  - Nr.2/2010
  - Nr.1/2010
  - Supplement 1/2010
- 2009
  - Nr.4/2009
  - Nr.3/2009
  - Nr.2/2009
  - Nr.1/2009
  - Supplement 1/2009
- 2008
  - Nr.4/2008
  - Nr.3/2008
  - Nr.2/2008
  - Nr.1/2008
  - Supplement 1/2008
- 2007
  - Nr.4/2007
  - Nr.3/2007
  - Nr.2/2007
  - Nr.1/2007
- 2006
  - Nr.4/2006
  - Nr.3/2006
  - Nr.2/2006
  - Nr.1/2006
- 2005
  - Nr.1/2005
Instructions for authors
Online applications
- Send an article
- Online payments
Editorial
About us
Links

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12

Top 10 downloaded articles - November 2024 -

Towards appropriate training f...	31
Recomandările naționale ale ...	28
Biomarkers of acute kidney inj...	23
A comprehensive review of glyc...	20
Agreement between different eG...	19
Investigation of cytokine chan...	19
Expressions of vascular endoth...	17
Role of Th1/Th2 imbalance medi...	16
Small patients, big challenges...	9
The frequency of EGFR gene mut...	8

Concept, Design & Programming
Dr. Adrian Man

Nr. 2(1)/2006

General Principles of Haematopoetic Stem Cell Transplantation. The Role of the Clinical Laboratory in High Quality Grafts Harvesting

Andreea Delia Moicean, Adriana Mariana Dumitrescu, Tatiana Puşcariu, Georgeta Niţu, Dana Ostroveanu, Mihaela Camelia Ghiţă, Virginia Nicoleta Mirea, Dan Nicolae Coliţă

Abstract:

The success of the haematopoetic stem cell transplantation (HSCT) depends on both the sensitivity of the malignant cells to chemotherapy and the quality of the graft. A high quality HSC graft that can ensure the recovery of the immune system and haematopoesis must be sterile and must provide at least 2.5 x 106 CD34+ positive cells /kg of recipient weight. The achievement of such a graft needs a team work between the HSCT group and performing laboratories. In the Center of Haematology and Bone Marrow Transplantation, Fundeni Clinical Institute, between March 2000 and December 2005, 47 grafts were collected with peripheral blood aphaeresis from 38 patients and 9 healthy donors. The following procedures were used: stem cell mobilization program, daily white blood cells (WBC) count, cytometry blood count of CD34+ stem cells, graft collecting, graft preparation for preservation and in vitro quality tests, cryopreservation in liquid nitrogen. In order to obtain 2.5x 106 CD34+ stem cells /kg recipient weight, 1 to 3 aphaeresis were required. The cell viability just after harvesting tested by direct means, was 94-99%. In vitro cell cultures showed 39 - 1074 x 104 colonies GM-CSF /kg weight/sample. Microbiological screening tests were negative for all samples. Before graft infusion, cell counting, viability tests, flow cytometry and stem cell cultures were repeated. 30 grafts were infused with the recovery of the haematopoesis in 7-15 days.

Keywords: haematopoetic stem cell transplantation,cytomorphology,flowcytometry,haematopoetic stem cell culture

Download full text PDF
(163 KB)

Moicean AD, Dumitrescu AM, Puşcariu T, Niţu G, Ostroveanu D, Ghiţă MC, et al. General Principles of Haematopoetic Stem Cell Transplantation. The Role of the Clinical Laboratory in High Quality Grafts Harvesting. Rev Romana Med Lab. 2006;2(1):48-54