RRML - Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction
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Nr. 30(2)/2022 DOI:10.2478/rrlm-2022-0018
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Research article

Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction

Arbab Husain, Sultan Alouffi, Afreen Khanam, Rihab Akasha, Saif Khan, Mahvish Khan, Alvina Farooqui, Saheem Ahmad

Correspondence should be addressed to: Alvina Farooqui, Saheem Ahmad

Abstract:

When glucose and Amadori products are auto-oxidized, glycation occurs, resulting in the formation of early (Amadori) and late advanced glycation end products (AGEs), as well as free radicals. Glycation and an increase in free radical activity induce diabetic complications. Antioxidant and antiglycation compounds may aid in the prevention of oxidation and glycation. The goal of this study was to assess the antiglycation and antioxidant capacity of C-phycocyanin (C-PC) derived from Plectonema sp. The DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide, hydroxyl radical scavenging activities and ferric ions reducing antioxidant power (FRAP) assays were used to assess antioxidant activity, while an in vitro bovine serum albumin-methyl glyoxal glycation (BSA-MG) model was used to assess glycation inhibitory potential. Glycation inhibition was measured using a variety of spectroscopic and biochemical parameters, including UV-visible & fluorescence spectroscopy, ketoamine, carbonyl and hydroxymethyl furfural content, as well as free lysine & free arginine estimations. In vitro, C-PC exhibited dose-dependent potent antioxidant activity, but lacked significant antiglycation potential. As a result, it is recommended that further studies be conducted to evaluate the antiglycation potential of C-PC.

Keywords: glycation, antioxidant, antiglycation, plectonema sp., C-phycocyanin, Bovine Serum Albumin (BSA), Methylglyoxal (MG)

Received: 25.11.2021
Accepted: 8.4.2022
Published: 15.4.2022

 
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How to cite
Husain A, Alouffi S, Khanam A, Akasha R, Khan S, Khan M, et al. Non-inhibitory effects of the potent antioxidant C-phycocyanin from Plectonema sp. on the in vitro glycation reaction. Rev Romana Med Lab. 2022;30(2):199-213. DOI:10.2478/rrlm-2022-0018