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Nr. 24(4)/2016 DOI:10.1515/rrlm-2016-0041
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Research article

Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn

Chuangpeng Shen, Liping Huang, Hua Xiang, Minzhen Deng, Huahong Gao, Zhangzhi Zhu, Min Liu, Guangbo Luo

Correspondence should be addressed to: Liping Huang

Abstract:

Cassia mimosoides Linn has been used from ancient times and used for treating hepatitis for its supposedly medically beneficial properties. In this study, different constituents of the Cassia mimosoides Linn (β-Sitosterol, Oleanolic Acid, Emodin, Carotene, Resorcinol, Luteolin, and α-L-Rhamnose) were evaluated for potential anti-HMG-CoA reductase effect. The inhibitory effects of HMG-CoA reductase of Cassia mimosoides Linn extracts and Pravastatin inhibitor at different concentrations (at doses of 1, 5, 25 or 125 μg/mL, respectively) in reaction system (70 mmol/L phosphate buffer, 200mmol/L NADPH, 5 μg HMG-CoA reductase, 2 mmol/L EDTA, 2 mmol/L cysteamine, 0.06% BSA) into 37°C preheat HMG-CoA for initiating this reaction, and then determined the change of HMG-CoA reductase activity (ΔAΔt) at 340 nm, the inhibition ratio of HMG-CoA reductase activity and its dynamic change of inhibitory effect within 15 min and the descent rate of NADPH. Emodin, Luteolin, β-Sitosterol, Oleanolic Acid, α-L-Rhamnose and Carotene showed good inhibition of HMG-CoA reductase activity. Among them, only the Emodin (1 and 5 μg/mL) groups showed a significant decrease of HMG-CoA reductase activity compared to the Pravastatin (1 and 5 μg/mL) groups respectively. In addition, the HMG-CoA reductase activity in the Emodin and Luteolin (25 and 125 μg/mL) groups was clearly lower than the Pravastatin (25 and 125 μg/mL) groups respectively. And the Emodin and Luteolin (1, 5, 25 or 125 μg/mL) groups exhibited a stable effect on inhibiting the HMG-CoA reductase within 15 min. These findings further support the exploration of Cassia mimosoides Linn as a potential agent for the treatment of hepatitis in future studies.

Keywords: Cassia mimosoides Linn, 3-Hydroxy-3-methylglutaryl-CoA reductase, Emodin, Luteolin, Chinese herbal medicines

Received: 31.1.2016
Accepted: 22.10.2016
Published: 3.12.2016

 
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How to cite
Shen C, Huang L, Xiang H, Deng M, Gao H, Zhu Z, et al. Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn. Rev Romana Med Lab. 2016;24(4):413-22. DOI:10.1515/rrlm-2016-0041