RRML - Non-invasive Sampling for Assessment of Oxidative Stress and Pro-inflammatory Cytokine Levels in Beta-Thalassaemia Major Patients
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English


Journal Metrics

Impact Factor 0.493
Five Year Impact Factor 0.531
SNIP 0.373
JCI 0.17


Advanced search


Top 10 downloaded articles
- December 2022 -
 
Downregulation of hsa-miR-4328... 5
Normality assessment, few para... 4
Setting up an own laboratory p... 3
The relationship between matri... 2
New Para-Clinical Investigatio... 2
New high-throughput liquid chr... 2
General Principles of Haematop... 2
Genetic risk factors for throm... 2
The importance of the new prog... 2
Detection of chromosomal imbal... 2

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 24(1)/2016 DOI:10.1515/rrlm-2016-0010
XML
TXT

Research article

Non-invasive Sampling for Assessment of Oxidative Stress and Pro-inflammatory Cytokine Levels in Beta-Thalassaemia Major Patients

Mohd. Rashdan Abd. Rahim, Jin Ai Mary Anne Tan, Ravishankar Ram Mani, Umah Rani Kuppusamy

Correspondence should be addressed to: Umah Rani Kuppusamy

Abstract:

Background: Beta-thalassaemia (β-thalassaemia) major patients are severely anaemic and require life-long blood transfusions for survival. These patients require iron-chelation therapy as a result of iron overload due to the monthly blood transfusions. The iron over load can cause oxidative damage and pro-inflammation and therefore, hasten mortality. Thus, regular monitoring of the oxidative stress and pro-inflammation status may be useful in these patients. Methods: Measurement of biomarkers is usually performed on serum samples but the evaluation in non-invasive samples such as saliva would be more favourable in paediatric cases. In this study, the levels of pro-inflammatory cytokines such as tumour necrosis factor- α (TNF-α) and interleukin-6 (IL-6) as well as oxidative indices such as lipid hydro peroxide, advanced oxidation protein products (AOPP), ferric reducing antioxidant power (FRAP), uric acid (UA) and glutathione peroxidase (GPx) activity in a total of 65 β-thalassaemia major patients (all on iron chelation) and 55 healthy control subjects were assessed. All the above biochemical parameters, measured using well established assay techniques, were detectable in saliva samples. Results: Non parametric analyses showed that lipid hydroperoxide (LH) and glutathione peroxidase (GPx) activities were significantly higher in β-thalassaemia major patients. All other parameters were not significantly different between patient and control groups implying that iron chelation therapy was successful in attenuating oxidative stress. Strong positive correlation was observed between FRAP and UA levels. There was also a notable difference in tumour necrosis factor- α (TNF-α) between the patients and healthy controls when analysed according to ethnicity and age. AOPP level in β+-thalassaemia homozygous patients were significantly higher than β+/β0-compound heterozygous and β0-thalassaemia homozygous patients. Conclusion: Saliva may serve as a reliable, non-invasive sample which can be used to assess oxidative indices and pro-inflammatory cytokines in β- thalassaemia major patients.

Keywords: β-thalassaemia major;saliva;oxidative stress;biochemical analysis

Received: 11.10.2015
Accepted: 11.2.2016
Published: 14.3.2016

 
  PDF Download full text PDF
(947 KB)
     
 
How to cite
Rahim MRA, Tan JAMA, Mani RR, Kuppusamy UR. Non-invasive Sampling for Assessment of Oxidative Stress and Pro-inflammatory Cytokine Levels in Beta-Thalassaemia Major Patients. Rev Romana Med Lab. 2016;24(1):83-92. DOI:10.1515/rrlm-2016-0010