RRML - From primer design to validation of results - is it possible by using free software only?

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Concept, Design & Programming
Dr. Adrian Man

Nr. 23(2)/2015 DOI:10.1515/rrlm-2015-0022

From primer design to validation of results - is it possible by using free software only?

Adrian Man, Claudia Bănescu, Minodora Dobreanu, Cornel Fraefel

Abstract:

Successful experiments in molecular biology require good knowledge about various methods and protocols. In molecular biology, nucleic acid manipulation is the essence, starting with the quality of extraction and ending with several analysis assays (PCR, RT-PCR, qPCR, PCR arrays, molecular cloning, etc). Though many of these are so called “standardized”, in practice there are many variables that can influence the outcome of the experiment. Due to the importance of optimal primer design in PCR assays, we will focus on primer designing and checking software, but we also present other useful free tools that can help researchers in the molecular biology field.

Keywords: primer design, free software, molecular biology

Received: 3.5.2015
Accepted: 6.6.2015
Published: 12.6.2015

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Man A, Bănescu C, Dobreanu M, Fraefel C. From primer design to validation of results - is it possible by using free software only?. Rev Romana Med Lab. 2015;23(2):155-8. DOI:10.1515/rrlm-2015-0022