RRML - Detection of the genetically modified organisms from food products

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Concept, Design & Programming
Dr. Adrian Man

Nr. 22(3)/2014 DOI:10.2478/rrlm-2014-0026

Detection of the genetically modified organisms from food products

Manuela Curticăpean, Augustin Curticăpean

Abstract:

Since the release of the first genetically modified crop, scientists have pro and con opinion on cultivation and their use, because of potential health, and environmental risks. Current EU legislation (Directive 2003/18/EC) provides for public information, monitoring long-term effects, labelling and traceability at all stages of the placing on the market of GMOs. The aim of this study was to evaluate the quality of the food products on the market, in which the detection of presence / absence of GMO. In this regard, two types of maize flour and four types of soy products were analysed during 2013. The kit used for detection of the presence / absence of GMO in the samples tested, comprising the step of DNA extraction, DNA amplification by PCR and agarose gel electrophoresis of amplified products and uses two GM associated sequences - promoter 35S and NOS terminator from Agrobacterium tumefaciens. The presence of GMO was found in a corn sample, also in a soy sample the results illustrate the need for further analysis to identify the exact type and quantity of GMO (the limiting value imposed by European legislation being 0.9% at ingredient).

Keywords: screening, GMO testing, PCR, food products

Received: 9.4.2014
Accepted: 29.6.2014
Published: 5.9.2014

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Curticăpean M, Curticăpean A. Detection of the genetically modified organisms from food products. Rev Romana Med Lab. 2014;22(3):387-95. DOI:10.2478/rrlm-2014-0026