RRML - Multiplex PCR approach for detection of fusion genes in acute lymphoblastic and myeloid leukemia
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Impact factor (2019): 0.945

Rejection rate (2016): 55%

Română English


Clarivate analytics (ISI) Impact factor


Advanced search


Top 10 downloaded articles
- October 2020 -
 
Romanian Review of Laboratory ... 16
High frequency of BRCA recurre... 12
Metabolomic biomarkers of poly... 10
Antibacterial and antitumor ac... 10
Troubleshootings in RNA extrac... 9
Evaluation of oxidative stress... 8
Assessment of circulating tumo... 8
The association of anthropomet... 7
Validation of GOD / PAP method... 6
Carbapenemase Producing Entero... 6

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 20(1)/2012
XML
TXT

Multiplex PCR approach for detection of fusion genes in acute lymphoblastic and myeloid leukemia

Dumitru Jardan, Rodica Talmaci, Cerasela Jardan, Daniel Coriu, Anca Colita, Andrei Colita, Constantin Arion


Abstract:

Acute leukemias are a group of heterogeneous diseases with respect to clinical features and acquired genetic aberrations. Since the discovery of BCR-ABL fusion gene a lot of other fusion genes were and are continuing to be discovered in leukemia patients. Many of these fusion genes are believed to be pathognomonic to disease and can be used as prognostic factors for the evolution of disease. In this study we performed development and optimization of a multiplex PCR assay for detection of 8 most common fusion genes in AML and ALL, namely: PML-RARα, AML1-ETO, CBFβ-MYH11, BCR-ABL, MLL-AF4, E2A-PBX1, SIL-TAL and TEL-AML1.Results: Our multiplex PCR assay allowed multiplexing of primers for detection of 8 most common fusion genes in acute leukemia. This allowed risk stratification for 36.5% of the patients. Furthermore identification of these fusion genes allowed MRD monitoring using nested PCR or real-time PCR for quantitative evaluation of MRD. Overall a very good performance was obtained with no non-specific amplifications and 100% correlation with commercial HemaVision kits. In comparison with HemaVision kits this assay allows identification of the most frequent 8 fusion genes at a considerably lower price per patient.

Keywords: Fusion genes,multiplex RT-PCR,acute leukemia

 
  PDF Download full text PDF
(98 KB)
     
 
How to cite
Jardan D, Talmaci R, Jardan C, Coriu D, Colita A, Colita A, et al. Multiplex PCR approach for detection of fusion genes in acute lymphoblastic and myeloid leukemia. Rev Romana Med Lab. 2012;20(1):15-20