RRML - Detection of mecA gene in clinical Staphylococcus aureus isolates from Infectious Diseases Hospital, Iasi, Romania
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Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 19(3)/2011
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Detection of mecA gene in clinical Staphylococcus aureus isolates from Infectious Diseases Hospital, Iasi, Romania

Olivia S. Dorneanu, Teodora Vremeră, Eduard V. Năstase, Cătălina Logigan, Aida C. Bădescu, Egidia G. Miftode


Abstract:

Introduction. Since it was first described in 1961, methicillin-resistant Staphylococcus aureus (MRSA) has been an increasingly important cause of health care-associated infections worldwide. During the 1990s, new strains of MRSA emerged and caused community-associated infections on multiple continents. Methods. We have investigated 151 clinically significant S. aureus strains isolated from blood cultures, cerebro-spinal fluid, pus, sputum samples collected from patients with infections admitted to the Infectious Diseases Hospital “Sf. Parascheva” Iaşi, Romania, between January 2008 and December 2009. We have performed real-time PCR for mecA gene detection, disk diffusion susceptibility testing and minimum inhibitory concentration determination to anti-staphylococcal antibiotics for all isolates. Results. The oxacillin resistance rate was 45.7%. All MRSA strains were susceptible to vancomycin, linezolid and teicoplanin; however, they exhibited high rates of resistance to erythromycin (70.5%), tetracycline (67.6%), gentamicin (48.5%) and ciprofloxacin (44.1%). Susceptibility rate to trimethoprim/sulfamethoxazole in MRSA strains was 89.7%. Almost a third of the tested strains had oxacillin MICs 256 µg/ml. For 90% of the strains vancomycin MICs was 2 µg/ml. Conclusions. Glycopeptides remain the first choice therapy for MRSA infections. To preserve their value, their use should be limited to those cases where they are clearly needed.

Keywords: Staphylococcus aureus,mecA,resistance

 
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How to cite
Dorneanu OS, Vremeră T, Năstase EV, Logigan C, Bădescu AC, Miftode EG. Detection of mecA gene in clinical Staphylococcus aureus isolates from Infectious Diseases Hospital, Iasi, Romania. Rev Romana Med Lab. 2011;19(3):259-65