RRML - Phylogenetic background and extraintestinal virulence genotypes of Escherichia coli vaginal strains isolated from adult women

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Nr. 19(1)/2011

Phylogenetic background and extraintestinal virulence genotypes of Escherichia coli vaginal strains isolated from adult women

Codruţa-Romaniţa Usein, Laura Andrea, Raluca Margareta, Mãdãlina, Maria Condei, Monica Delia

Abstract:

Distinctive Escherichia coli clones, designated as extraintestinal pathogenic E. coli (ExPEC), are responsible for extraintestinal infections. Human vagina may be a transient residence for E. coli and its immediate source for adjoining sites such as the urinary tract. The aim of this study was to complement the routine microbiological diagnostic by examining whether the E. coli isolates recovered from vaginal specimens possessed ExPEC-associated traits. Ninety-three E. coli isolates were investigated using PCR-based protocols for their phylogenetic origin and virulence genotype, targeting 14 virulence genes. Genetic relatedness among the isolates was assessed using PFGE of XbaI macrorestriction DNA fragments. Phylogenetic groups A, B1, B2, and D accounted for 20%, 2%, 65%, and 13%, respectively. At least one virulence gene region was detected in each examined isolate, their prevalence ranging from 0% (papGI) to 98% (fimH). Group B2-derived isolates, found as diverse based on the PFGE profiles, exhibited the highest virulence content. Overall, certain of the sought genes, e.g. fyuA (85%), irp2 (85%), sfa/focDE (53%), hly (52%), iucC (51%), cnf1 (46%), and papC (42%) were more frequently detected in the examined isolates than others, e.g. sat (17%), ibeA (17%), and afaC (5%). As for papG alleles, papGIII occurred more frequently than papGII. The genetic analysis allowed to distinguish among the vaginal E. coli isolates those which possessed an assortment of virulence genes that can promote an infectious process. However, much further work remains to be done before deciding whether this can become feasible in laboratory routine practice.

Keywords: vaginal E. coli,ExPEC,extraintestinal virulence genes

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Usein CR, Andrea L, Margareta R, Mãdãlina , Condei M, Delia M. Phylogenetic background and extraintestinal virulence genotypes of Escherichia coli vaginal strains isolated from adult women. Rev Romana Med Lab. 2011;19(1):37-45