RRML - Changes in the erythrocyte membrane cytoskeleton in microcytic hemolytic anemia – case report
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2016): 55%

Română English


SCImago Journal & Country Rank


Advanced search


Top 10 downloaded articles
- February 2020 -
 
The role of neutrophil to lymp... 16
The direct deleterious effect ... 16
The usefulness of neutrophil/l... 14
Quality Control Strategy for A... 11
Genetic testing in pediatrics ... 9
Is there a correlation between... 7
Variability of ex-vivo stimula... 7
A rare case of acute myeloid l... 7
Laboratory medicine in the era... 6
HBV genotypes circulation in p... 6

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 18(3)/2010
XML
TXT

Changes in the erythrocyte membrane cytoskeleton in microcytic hemolytic anemia – case report

Horea Matei, Adrian Florea, Adina Chiş, Gheorghe Popa, Mariana Tilinca


Abstract:

A case of microcytic anemia from the Cluj-Napoca Pediatric Hospital is presented. Light and scanning electron microscopy data indicate poikilocytosis (with a high ratio of spherocytes – but irregular-shaped cells are also present), and anisocytosis (with a high number of microcytes, many cells having diameters under 4 µm). The protein composition of the erythrocyte membranes was analysed using SDS-PAGE and the percentages of proteins in the various bands were analysed by videodensitometry. A spectrin/band 3 subunitary ratio was observed, suggesting a quantitative deficit of spectrin. The ankyrin/band 3 ratio was also reduced, under the limit of 0.2, indicating defects at the ankyrin level as well. The results of our study proved that defects in both spectrin and ankyrin from the erythrocyte membrane cytoskeleton are the main cause of microcytic anemia in this case.

Keywords: erythrocyte,spectrin,ankyrin,microcytic anemia,SDS-PAGE

 
  PDF Download full text PDF
(4496 KB)
     
 
How to cite
Matei H, Florea A, Chiş A, Popa G, Tilinca M. Changes in the erythrocyte membrane cytoskeleton in microcytic hemolytic anemia – case report. Rev Romana Med Lab. 2010;18(3):31-7