RRML - Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English


Journal Metrics

Impact Factor 0.493
Five Year Impact Factor 0.531
SNIP 0.373
JCI 0.17


Advanced search


Top 10 downloaded articles
- December 2022 -
 
Downregulation of hsa-miR-4328... 5
Normality assessment, few para... 4
Setting up an own laboratory p... 3
The relationship between matri... 2
New Para-Clinical Investigatio... 2
New high-throughput liquid chr... 2
General Principles of Haematop... 2
Genetic risk factors for throm... 2
The importance of the new prog... 2
Detection of chromosomal imbal... 2

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 18(2)/2010
XML
TXT

Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer

Denisa Anderco, Elena Lazăr, Sorina Tăban, Felix Mic, Angela Borda, Andrada Loghin, Alis Dema


Abstract:

Prostate biopsy is the standard procedure used in the diagnosis of prostate cancer. Immunostains, additional to standard stains, are sometimes necessary for correct interpretation and classification of atypical, minute lesions. However, immunohistochemistry becomes noncontributory in the situation of loss of suspected foci after repeated sections from the paraffin block. This situation, which is not at all infrequent in prostate pathology, led to the design of special techniques that make immunostaining possible. We further present the results of an innovative technique of tissue protection immunohistochemistry which allows the immunostaining on an initially H&E-stained slide. Unlike other methods described before, this technique does not require H&E destaining prior to session and did not involve saving intervening unstained sections for possible immunohistochemical examination. The great advantage of tissue protection immunohistochemistry is that it allows simultaneous examination of H&E and immunostaining on the same slide. It only requires that the original H&E-stained sections to be placed on charged slides.

Keywords: technique,prostate biopsy,diagnosis,immunohistochemistry

 
  PDF Download full text PDF
(425 KB)
     
 
How to cite
Anderco D, Lazăr E, Tăban S, Mic F, Borda A, Loghin A, et al. Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer. Rev Romana Med Lab. 2010;18(2):63-9