RRML - Peripheral and uterine cytotoxicity in normal and pathologic first trimester pregnancies

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English

Journal Metrics

Impact Factor 0.493
Five Year Impact Factor 0.531
SNIP 0.373
JCI 0.17

Advanced search

Top 10 downloaded articles
- December 2022 -
Downregulation of hsa-miR-4328... 5
Normality assessment, few para... 4
High DNAJA4 expression correla... 4
Setting up an own laboratory p... 4
New Para-Clinical Investigatio... 3
Genetic risk factors for throm... 3
miR-190, CDK1, MCM10 and NDC80... 3
The relationship between matri... 2
A model for calculating measur... 2
The Use of Infrared Spectrosco... 2

Log in

Concept, Design & Programming
Dr. Adrian Man

Nr. 11(2)/2008

Peripheral and uterine cytotoxicity in normal and pathologic first trimester pregnancies

Daniela Constantinescu, Carmen Cozmei, Andreea Chiriac, Elena Mihălceanu, Irina Dumitraşcu, Eugen Carasevici


The maternal immune system is involved in pregnancy success or failure. Increased cytotoxic activity of NK cells was associated with first trimester abortion, which is the most frequent complication of pregnancy. The aim of our study was to compare peripheral and uterine cytotoxicity in normal and pathological first-trimester pregnacy, in order to evaluate the utility of this test in monitoring high-risk pregnancies. Material and methods: mononuclear cells were separated from peripheral blood and from tissue fragments obtained by uterine curretage from 10 pregnant women with requested abortion and 10 women with spontaneous abortion. Natural-killer (NK) cells percentage was detected by flow-cytometry. Separation by triple gradient Percoll density centrifugation allowed simultaneous isolation of trophoblast cells from uterine tissue fragments. We used a flowcytometric method of cytotoxicity evaluation which used carboxy-fluorescein-succinimidyl ester (CFSE)-labelled K562 and autologous trophoblastic cells as targets. Results: cytotoxic activity of uterine cells was higher than that of peripheral blood cells. Cytotoxicity was reduced in pathological compared to normal pregnancy. Peripheral blood and uterine cytotoxicity indexes were significantly different and were correlated only in normal pregnancies. The percentage of NK cells did nor correlate with their cytotoxic activity. Conclusions: increased cytotoxic activity cannot be incriminated in inducing abortion. The difference between the cytotoxic activity of uterine and peripheral blood cells proves the compartmentalisation of uterine events.

Keywords: cytotoxicity,K562,trophoblast,CFSE,NK

  PDF Download full text PDF
(151 KB)
How to cite
Constantinescu D, Cozmei C, Chiriac A, Mihălceanu E, Dumitraşcu I, Carasevici E. Peripheral and uterine cytotoxicity in normal and pathologic first trimester pregnancies. Rev Romana Med Lab. 2008;11(2):7-13