RRML - Assessment of circulating tumor cells in peripheral blood using flow cytometry in patients with surgery for colorectal cancer – review
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Nr. 28(4)/2020 DOI:10.2478/rrlm-2020-0033
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Review

Assessment of circulating tumor cells in peripheral blood using flow cytometry in patients with surgery for colorectal cancer – review

Ana-Maria Mușină, Ionuț Huțanu, Mihaela Zlei, Mădălina Ștefan, Mihaela Mentel, Maria-Gabriela Aniței, Bogdan Filip, Dragoș Viorel Scripcariu, Mihaela-Mădălina Gavrilescu, Andrian Panuța, Mihaela Buna-Arvinte, Iulian Radu, Viorel Scripcariu

Correspondence should be addressed to: Ionuț Huțanu, Dragos Viorel Scripcariu

Abstract:

Introduction: Colorectal cancer (CRC) is the third most common neoplasia in the world. Circulating tumor cells (CTC) have a prognostic value and can be useful in monitoring solid neoplasia. Only one method for CTC identification has received the approval and this is the CellSearch® system based on the immunomagnetic separation. Multiple markers are used in CTC identification, as epithelial markers and cytokeratines. CTC identification in peripheral blood is associated with a worse prognostic and reduced free survival in CRC. Material and methods: We performed a systematic search in PubMed database for articles that reports the circulating tumor cells in CRC until July 2019. We selected studies in English and French and the main words used for search were ‘circulating tumor cells’, ‘colorectal cancer’, ‘colon cancer’, ‘rectal cancer’, ‘flow cytometry’, ‘peripheral blood’. We included studies with more than 10 patients, where samples were collected from the blood in relation with surgery and flow cytometry was used as analyzing technique. Results: We included 7 studies in final analysis, that showed in flow cytometry analysis a cut-off value of CTC that can vary from 2-4 CTC/ 7.5 ml peripheral blood with a sensitivity of 50.8% and specificity of 95%. Patients with positive CTC were associated with higher T stage and positive lymph nodes, with a worse overall survival (OS) and disease free survival (DFS) comparing with negative patients. Conclusion: CTC are considered to be a prognostic factor who needs more validation studies in order to be included in the clinical practice.

Keywords: colorectal cancer, flow cytometry, circulating tumor cells

Received: 29.6.2020
Accepted: 11.9.2020
Published: 22.9.2020

 
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How to cite
Mușină AM, Huțanu I, Zlei M, Ștefan M, Mentel M, Aniței MG, et al. Assessment of circulating tumor cells in peripheral blood using flow cytometry in patients with surgery for colorectal cancer – review. Rev Romana Med Lab. 2020;28(4):365-79. DOI:10.2478/rrlm-2020-0033