RRML - Implantable orthotopic bladder cancer model in Wistar rats: A pilot and feasibility study
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2016): 55%

Română English


SCImago Journal & Country Rank


Advanced search


Top 10 downloaded articles
- July 2019 -
 
Simultaneous FLT3, NPM1 and DN... 12
Do we really need genetic test... 8
Genotype comparison of Candida... 8
Laboratory medicine in the era... 5
Antibiotic susceptibility and ... 4
Point-of-care testing in diabe... 4
Antibacterial activity of sele... 4
Multiplex ligation dependent p... 3
Expanding PVL positive MRSA He... 3
The utility of molecular genet... 3

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 27(1)/2019 DOI:10.2478/rrlm-2019-0002
XML
TXT

Research article

Implantable orthotopic bladder cancer model in Wistar rats: A pilot and feasibility study

Kerem Teke, Hasan Yilmaz, Mehmet Esad Kosem, Mustafa Yuksekkaya, Sema Yusufoglu, Busra Yaprak Bayrak, Yusufhan Yazir, Demir Kursat Yildiz, Ozdal Dillioglugil

Correspondence should be addressed to: Kerem Teke

Abstract:

Purpose: The implantable bladder cancer (BC) models allow the researchers to perform rapid and useful experiments for BC. We investigated the implantation success of BC cells obtained from Wistar rats (grown in vitro), into bladders of syngeneic Wistar rats, which are commonly used in the laboratories. Methods: The Nara Bladder Tumor No.2 (NBT-II) BC cells induced with 4-hydroxybutylnitrosamine were grown with passages in Kocaeli University Center for Stem-Cell and Gene-Therapies. After urothelial denudation, 2x106 NBT-II cells were then implanted into bladders of 24 female Wistar rats (aged 7-8 weeks). The rats were randomly divided into four experimental groups; three instillation groups (8 per group) and one sham-operated control group consisting of 6 rats. First, second and third instillation groups were sacrificed at days 7, 14, and 21, respectively, and, bladders were histopathologically evaluated for BC according to WHO / International Society of Urological Pathology. Results: All tumors were pT1 (including 1 rat that prematurely died at 5th day), except one rat that died prematurely at 8th day had pT2 tumor. Implantation rates were 28.58% (2/7) in the first group, and 42.85% (3/7) in the second, for a cumulative rate of 35.71% (5/14) in these two-groups (until 14th day). Interestingly, there was no tumor in the third group, but there was an inflammatory granulation tissue. Conclusion: Seeding NBT-II cells into bladders of Wistar rats was described, successfully tested and demonstrated in this study. This implantable BC model of Wistar rats may be improved to increase the success rate of BC cell implantation in new studies with higher number of animals.

Keywords: bladder cancer model, implantable, orthotopic, syngeneic, Wistar rats

Received: 17.6.2018
Accepted: 27.10.2018
Published: 12.11.2018

 
  PDF Download full text PDF
(749 KB)
     
 
How to cite
Teke K, Yilmaz H, Kosem ME, Yuksekkaya M, Yusufoglu S, Bayrak BY, et al. Implantable orthotopic bladder cancer model in Wistar rats: A pilot and feasibility study. Rev Romana Med Lab. 2019;27(1):35-42. DOI:10.2478/rrlm-2019-0002